Ultrastructures of Frozen-thawed Boar Spermatozoa
نویسندگان
چکیده
منابع مشابه
Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa.
The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-...
متن کاملEffect of processing of semen on capacitation time of fresh and frozen-thawed boar spermatozoa.
Ovulation time of gilts was controlled by oral treatment with altrenogest (17-allyl-hydroxyestra-4,9,11-trien-3-one) followed by gonadotropic hormone injections. Gilts were laparotomized 42 to 44 h after injection of human chorionic gonadotropin. Spermatozoa were surgically placed into ligated oviducts of gilts after one of the following semen treatments: frozen and thawed in Beltsville thawing...
متن کاملComparative Study of Different Methods for Plasma Membrane Integrity Assessment of Frozen-thawed Boar Spermatozoa
In this study, different assays were used to assess the structural and functional integrity of the sperm plasma membrane following freezing-thawing of the whole ejaculates (WEs) and sperm-rich fractions (SRFs) of boar semen. Besides sperm viability assessments (motility and mitochondrial membrane potential using a mitochondrial specific dye, JC-1 with propidium iodide, PI), sperm plasma membran...
متن کاملFrozen boar spermatozoa: methods of thawing pellets.
In four experiments, the effects of pre-thaw delay, method of thawing, temperature of thawing solution and pellet volume on acrosome morphology and moti l i ty of" boar spermatozoa frozen by the pellet method were examined. Holding the frozen pellets for 0, 1.5, 3 or 4.5 min in a Styrofoam container before transferring them into the thawing solution resulted in maximum post-thaw percentages of ...
متن کاملImproved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy
Genetically modified (GM) animals are unique mutants with an enormous scientific potential. Cryopreservation of pre-implantation embryos or spermatozoa is a common approach for protecting these lines from being lost or to store them in a repository. A mutant line can be taken out of a breeding nucleus only if sufficient numbers of samples with an appropriate level of quality are cryopreserved. ...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 1995
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.95-415j9